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Antibody Screening Test
Blood Culture
Bone Scan
Cardiac Blood Pool Imaging
Complement Assays
Contraction Stress Test
Direct Antiglobulin Test
Hepatitis B Surface Antigen
Herpes Simplex Antibodies
Human Chorionic Gonadotropin
Liver Spleen Scanning
Pelvic Ultrasonography
Percutaneous Renal Biopsy
Percutaneous Transhepatic Cholangiography
Raji Cell Assay
Renal Ultrasonography
Respiratory Syncytial Virus Antibodies
Skin Biopsy
T-And B-Lymphocyte Assays
Ultrasonography of the Spleen
Wound Culture

Raji Cell Assay

This assay, which is performed to detect the presence of circulating immune complexes, studies the Raji lymphoblastoid cell line. Identifying these cells, which have receptors for immunoglobulin G complement, helps to evaluate autoimmune disease.


  • To detect circulating immune complexes
  • To aid the study of autoimmune disease

Patient preparation

  • As appropriate, explain to the patient the purpose of the test.
  • Tell him that the test requires a blood sample and who will perform the venipuncture and when.
  • Reassure him that although he may experience transient discomfort from the needle puncture and the tourniquet, collecting the sample takes less than 3 minutes.

Procedure and posttest care

  • Perform a venipuncture, and collect a sample in a red-top, red-marble-top, or green-top tube. Place the sample on ice and send it to the laboratory for assay.
  • If a hematoma develops at the venipuncture site, apply warm soaks.


  • Handle the sample gently to prevent hemolysis.

Reference values

Normal value is less than 13 mg AHG (antihemophilic globulin) Eq/ml.

Abnormal findings

A positive Raji cell assay can detect immune complexes, including those found in viral, microbial, and parasitic infections; metastasis; autoimmune disorders; and drug reactions. This test may also detect immune complexes associated with celiac disease, cirrhosis, Crohn's disease, cryoglobulinemia, dermatitis herpetiformis, sickle cell anemia, and ulcerative colitis.

Interfering factors

  • Hemolysis due to rough handling of the sample
  • Recent injection of radioactive contrast medium
  • Failure to place the sample on ice

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